中国医学科学院医药生物技术研究所

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The MOV10 helicase inhibits LINE-1 mobility.
来源: 时间:2014-07-07 浏览次数:3711
J Biol Chem. 2013 Jul 19;288(29):21148-60. doi: 10.1074/jbc.M113.465856. Epub 2013 Jun 10.
The MOV10helicaseinhibitsLINE-1mobility.
  • Institute of Medicinal Biotechnology, Chinese Academy of Medical Science, Beijing 100050, China.
Abstract
LINE-1 (long interspersed element 1) is an autonomous non-long terminal repeat retrotransposon. Its replication often causes mutation and rearrangement of host genomic DNA. Accordingly, host cells have evolved mechanisms to control LINE-1 mobility. Here, we report that a helicase named MOV10 effectively suppresses LINE-1 transposition. Mutating the helicase motifs impairs this function of MOV10, suggesting that MOV10 requires its helicase activity to suppress LINE-1 replication. Further studies show that MOV10 post-transcriptionally diminishes the level of LINE-1 RNA. The association of MOV10 with both LINE-1 RNA and ORF1 suggests that MOV10 interacts with LINE-1 RNP and consequently causes its RNA degradation. These data demonstrate collectively that MOV10 contributes to the cellular control of LINE-1 replication.
KEYWORDS:
Host-Pathogen Interactions, LINE-1, MOV10, Post-transcriptional, RNA Helicase, Restriction, Retrotransposon, Reverse Transcription, Viral Replication, Virology